Effectiveness of a Genital Hygiene E-Guide in Improving Knowledge, Attitudes, and Practices Related to Urinary Tract Infection Prevention Among Adolescent Girls
Abstract
Urinary tract infections (UTIs) are common among adolescent girls and are strongly associated with anatomical characteristics and inadequate genital hygiene practices. Digital educational interventions may improve preventive health behaviors by enhancing knowledge, attitudes, and hygiene practices. This study aimed to evaluate the effectiveness of a genital hygiene e-guide on knowledge, attitudes, and practices related to urinary tract infection prevention among adolescent girls. A quasi-experimental one-group pretest-posttest study was conducted among 32 female students in a senior high school selected through purposive sampling in Blora Regency, Indonesia. Participant received a web-based genital hygiene e-guide, and knowledge, attitudes, and practices were assessed using a structured questionnaire before and after the intervention. Paired t-tests were performed with a significance level of p<0.05, 95% confidence interval, and effect sizes were calculated using Cohen's d. Following the intervention, significant improvements were observed in knowledge (47.19±8.13 vs. 94.38±6.19), attitude (51.75±7.39 vs. 91.16±3.09), and practice (49.38±6.19 vs. 91.72±2.79) (all (p<0.001). Very large effect sizes were observed for knowledge (d=6.53), attitudes (d=6.96), and practices (d=7.98). In conclusion, the genital hygiene e-guide is highly effective in improving knowledge, attitudes, and practices among adolescent girls. This digital-based intervention has strong potential as a promotive and preventive strategy to reduce the risk of UTIs and can be utilized as an accessible health education tool in school settings. Further controlled studies with larger populations are warranted to confirm these findings.
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References
- 1. Mancuso G, Midiri A, Gerace E, Marra M, Zummo S, Biondo C. Urinary Tract Infections: The Current Scenario and Future Prospects. Pathogens. 2023 Apr;12(4):623. doi:10.3390/pathogens12040623
- 2. van Geelen H, Sand PK. The female urethra: urethral function throughout a woman’s lifetime. Int Urogynecology J. 2023 Jun 1;34(6):1175–86. doi:10.1007/s00192-023-05469-6
- 3. Elendu C, Amaechi DC, Elendu ID, Elendu TC, Amaechi EC, Usoro EU, et al. Global perspectives on the burden of sexually transmitted diseases: A narrative review. Medicine (Baltimore). 2024 May 17;103(20):e38199. doi:10.1097/MD.0000000000038199
- 4. Elorfaly HMA. The Relation between Genital Hygiene Behaviors in Women and Urinary Tract Infection in Any Period of Life: Review Article. Egypt J Hosp Med. 2024 Oct 1;97(1):3811–9. doi:10.21608/ejhm.2024.390051
- 5. Sinaga CL, Situmeang IRVO. Infeksi Saluran Kemih dan Pencegahannya. J Med Methodist MediMeth. 2024;2(3):32–8.
- 6. Umami A, Paulik E, Molnár R, Murti B. The relationship between genital hygiene behaviors and genital infections among women: A systematic review. J Ners. 2022 Apr 25;17(1). doi:10.20473/jn.v17i1.34402
- 7. Kundu N, Mou FA, Samia FN, Urmi CY, Hossain MdS, Ahmad T. Knowledge-level, attitudinal-trend and preventive-practices on urinary tract infections among reproductive-aged female university students. Discov Public Health. 2025 Oct 1;22(1):587. doi:10.1186/s12982-025-00990-z
- 8. Naswa LA, Aisyaroh N. Pengaruh Edukasi Kesehatan dalam Mencegah Infeksi Saluran Kemih pada Remaja Putri: Literature Review. Sci-Tech J. 2025 Oct 5;4(2):281–93. doi:10.56709/stj.v4i3.710
- 9. Rusli M, Hermawan D, Supuwiningsih NN. Memahami E-learning: Konsep, Teknologi, dan Arah Perkembangan. Yogyakarta: Penerbit Andi; 2020. 114 p.
- 10. Hidayati PI, Arifin AS. PIH Pengaruh Model Pembelajaran Berbasis Masalah Melalui Pendekatan Ilmiah terhadap Pemahaman Konseptual Mahasiswa dalam Matakuliah Psikologi Pendidikan. Paradig J Filsafat Sains Teknol Dan Sos Budaya. 2026 Jan 13;32(1):50–9. doi:10.33503/paradigma.v32i1.2810
- 11. Alidousti K. Effect of integrated Health Promotion Intervention and follow up on Health issues (clothing way, food habits, urinary habits, sexual behavior habits) Related to Urinary Tract Infection Among Pregnant women. A Randomized, Clinical Trial. J Prev Med Hyg. 2020 May 12;61(2):E194–E194. doi:10.15167/2421-4248/jpmh2020.61.2.1412
- 12. Mohamed YA, Khanan A, Bashir M. An IoT-Enabled E-Guide System for Integrated Healthcare Center Navigation and Service Optimization in Oman. In: 2025 International Conference on Electrical and Computer Engineering Researches (ICECER) [Internet]. 2025 [cited 2026 Aug 20]. p. 1–13. Available from: https://ieeexplore.ieee.org/document/11401380 doi:10.1109/ICECER65523.2025.11401380
- 13. Indra I, Sartika S, Maryadi M, Purwanto E. Kebiasaan Menahan Kemih dan Kebersihan Genitalia Sebagai Faktor Risiko Infeksi Saluran Kemih. J Penelit Kesehat SUARA FORIKES J Health Res Forikes Voice. 2025 Jan 17;16(1):5–7. doi:10.33846/sf16102
- 14. Jagtap S, Harikumar S, Vinayagamoorthy V, Mukhopadhyay S, Dongre A. Comprehensive assessment of holding urine as a behavioral risk factor for UTI in women and reasons for delayed voiding. BMC Infect Dis. 2022 Jun 6;22(1):521. doi:10.1186/s12879-022-07501-4
- 15. Guo R, Xie H, Zhao W, Wang J. Web-based sexual and reproductive health education for adolescents aged 10–17 years: a systematic review and meta-analysis. BMJ Paediatr Open. 2025 Oct 31;9(1). doi:10.1136/bmjpo-2025-003714 PubMed PMID: 10.1136/bmjpo-2025-003714.
- 16. Aguilar-Quesada A, Sierra-Yagüe A, González-Cano-Caballero M, Zafra-Egea JA, Lima-Serrano M. Effectiveness of digital interventions to reduce school-age adolescent sexual risks: A systematic review. J Nurs Scholarsh Off Publ Sigma Theta Tau Int Honor Soc Nurs. 2025 Mar;57(2):342–53. doi:10.1111/jnu.13015 PubMed PMID: 39118266; PubMed Central PMCID: PMC11931981.
- 17. Haghdoost S, Pazandeh F, Khabazkhoob M, Behroozi Lak T. Association Between Sexual and Genital Hygiene Habits With the Urinary Tract Infection During Pregnancy: A Case-Control Study. Int J Womens Health Reprod Sci. 2019 Sep 9;8(2):158–64. doi:10.15296/ijwhr.2020.25
- 18. Mohamed Hassan Eltelt R, Mohamed Mohamed N, Abdel Hakeem Aboud S, Hussein Mohammed Aboelfottoh S. Effect of applying an Instructional System Design Model on Knowledge, Attitude and Satisfaction regarding Vaginal Infections among Female University Students. J Nurs Sci Benha Univ. 2026 Jan 1. doi:10.21608/jnsbu.2026.495274
- 19. Wigati LI, Suryati E. Infeksi Saluran Kemih Rekuren pada Anak Perempuan Usia 7 Tahun: Sebuah Laporan Kasus.
- 20. Njee RM, Imeda CP, Ali SM, Mushi AK, Mbata DD, Kapala AW, et al. Menstrual health and hygiene knowledge among post menarche adolescent school girls in urban and rural Tanzania. PLOS ONE. 2024 Mar 11;19(3):e0284072. doi:10.1371/journal.pone.0284072
- 21. Chen Y, Bruning E, Rubino J, Eder SE. Role of female intimate hygiene in vulvovaginal health: Global hygiene practices and product usage. Womens Health. 2017 Dec;13(3):58–67. doi:10.1177/1745505717731011 PubMed PMID: 28934912; PubMed Central PMCID: PMC7789027.
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