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Comparative Effectiveness of Green Betel Leaf (Piper betle L.) Spray Gel and Conventional Gel on Perineal Wound Healing in Mice (Mus musculus)

Monika Herti Kurniasari Poltekkes Kemenkes Semarang, Indonesia Correspondence Monikahertiks@gmail.com
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Arwani Arwani Poltekkes Kemenkes Semarang, Indonesia Correspondence arwani_arwani@ymail.com
,
Irmawati Irmawati Poltekkes Kemenkes Semarang, Indonesia Correspondence irmaartikel@gmail.com
Pages 103-111
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Abstract

Perineal wounds following vaginal delivery are common and may lead to pain, infection, and delayed healing, reduced maternal quality of life. Green betel leaves (Piper betle L.) possess antibacterial, anti-inflammatory, and antioxidant that may promote wound healing, while spray gel formulations offer hygienic application and improved drug distribution. This study compared the effectiveness of green betel leaf spray gel and conventional gel in promoting perineal wound healing. A true experimental pretest-posttest study was conducted using 36 female Mus musculus with induced perineal wounds randomly allocated to spray or conventional gel groups (n =18 each). Perineal wound healing was assessed using REEDA scores on days 1, 3, 5, and 7. Data were analyzed using Friedman and Mann–Whitney U tests. Both formulations significantly improved wound healing over time. However, the spray gel demonstrated significantly greater improvement than the conventional gel, with a higher mean ∆REEDA score (5.28 ± 0.575 vs 4.67 ± 0.840, p = 0.020), indicating greater reductions in wound severity.  Green betel leaf spray gel was more effective than conventional gel in promoting perineal wound healing in mice. Its hygienic, non-contact application and superior healing performance support its potential as a topical intervention for postpartum perineal care, warranting further clinical evaluation.

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How to Cite This

Kurniasari, M. H., Arwani, A., & Irmawati, I. (2026). Comparative Effectiveness of Green Betel Leaf (Piper betle L.) Spray Gel and Conventional Gel on Perineal Wound Healing in Mice (Mus musculus). Jurnal Kesehatan Ibu Dan Anak, 19(2), 103–111. https://doi.org/10.29238/kia.v19i2.3044

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